not in use here
16 Foundation for Applied Medical Research, ES
Role and Contribution
The preclinical participation of the group will consist in developing a technique to visualise in mice by positron emission tomography the fate of viable dendritic cells after injection through various routes (intra-tumoural, subcutaneous, intravenous, intraperitoneal, microsurgically intranodal). The idea will be to gene mark the dendritic cells with an adenovirus encoding thymidine kinase. Such enzyme in viable cells will phosphorylate a gancyclovir analogue marked that is retained inside the TK+ cells. Once this technique is in place the goal is to use it to monitor migration of dendritic cells under the influence of IFN and PolyI:C in mice. The advantage is that only the cells with a preserved membrane will retain the contrast, and thus it will be possible to monitor cell viability and migration at the same time.
Bsc and PhD in Biochemistry from Navarra University has been working in molecular biology of cancer for four years in the US with professor Notario (University of George Town). She provides expertise both on molecular biology and on chemotaxis.