not in use here
16 Foundation for Applied Medical Research, ES
Role and Contribution
The preclinical participation of the group will consist in developing a technique to visualise in mice by positron emission tomography the fate of viable dendritic cells after injection through various routes (intra-tumoural, subcutaneous, intravenous, intraperitoneal, microsurgically intranodal). The idea will be to gene mark the dendritic cells with an adenovirus encoding thymidine kinase. Such enzyme in viable cells will phosphorylate a gancyclovir analogue marked that is retained inside the TK+ cells. Once this technique is in place the goal is to use it to monitor migration of dendritic cells under the influence of IFN and PolyI:C in mice. The advantage is that only the cells with a preserved membrane will retain the contrast, and thus it will be possible to monitor cell viability and migration at the same time.
FIMA
FIMA
Staff
Ivan Martínez Forero
PhD. Ivan's undergraduate training was in Medicine, but his subsequent work towards his PhD was in Mathematical Biology and Systems Biology. He joined the laboratory of Immunology and Gene Therapy under the leadership of Prof. Ignacio Melero at the Centre for Applied Medical Research in Pamplona/ES. Responsibility within ENCITE: production of a clinical grade adenoviral vector expressing Thymidine Kinase. This vector would infect dendritic cells allowing it to be visualised by Positron Emission Tomography after injection trough various routes (intravenous, intranodal).
PhD. Ivan's undergraduate training was in Medicine, but his subsequent work towards his PhD was in Mathematical Biology and Systems Biology. He joined the laboratory of Immunology and Gene Therapy under the leadership of Prof. Ignacio Melero at the Centre for Applied Medical Research in Pamplona/ES. Responsibility within ENCITE: production of a clinical grade adenoviral vector expressing Thymidine Kinase. This vector would infect dendritic cells allowing it to be visualised by Positron Emission Tomography after injection trough various routes (intravenous, intranodal).